Recombinant:Article Title: Optogenetic mediated contractility enables reversible control of microglial morphology and migration in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins BODIPY 493/503 Thermo Fisher Scientific Cat#: D3922 N-Phenylthiourea (PTU) Sigma-Aldrich Cat#: P7629 Tricaine Sigma-Aldrich Cat#: A5040 CK-666 Sigma-Aldrich Cat#: SML0006 Blebbistatin MedChemExpress Cat#: HY-13441 Deposited data UAS:lyn-iLID-P2A-mScarlet-I-SspB-ArhGEF25b Addgene Cat#: 253351 Protrusion_morphology_seg_track_3D This study https://doi.org/10.5281/zenodo.18662631 Experimental models: Organisms/strains Danio rerio TgBAC(csf1ra:GAL4-VP16) Gray et al.55 ZFIN ID:ZDB-TGCONSTRCT-110707-1 Danio rerio Tg(mpeg1.1:GAL4-VP16) Ellet et al.56 ZFIN ID:ZDB-TGCONSTRCT-120117-3 Danio rerio Tg(UAS:lyn-tagRFPt) Möller et al.3 N/A Danio rerio Tg(NBT:ΔLexPR:LexOP:secA5-BFP) Mazaheri et al.2 ZFIN ID: ZDB-ALT-170110-1 Danio rerio TgBAC(p2y12:p2y12-GFP) Sieger et al.11 ZFIN ID: ZDB-TGCONSTRCT- 121109-1 Danio rerio Tg(UAS:Myl12.1-mNeonGreen) This study N/A Danio rerio Tg(UAS:LifeAct-mNeonGreen) This study N/A Danio rerio Tg(UAS:lyn-miRFP670) This study N/A Danio rerio Tg(UAS:lyn-iLID-P2AmScarlet-I-SspB-ArhGEF25b) This study N/A Software and algorithms FIJI Schindelin et al.57 N/A BigDataProcessor2 Tischer et al.58 N/A BioFormats Linkert et al.59 N/A Linear Stack Alignment with SIFT Fischler and Bolles60 Lowe61 N/A MTrackJ Meijering et al.62 N/A Trainable WEKA Segmentation Arganda-Carreras et al.63 N/A Huygens Professional SVI svi.nl/Huygens-Professional Ilastik Berg et al.64 N/A Imaris Oxford Instruments imaris.oxinst.com RStudio R project r-project.org Python Python python.org SciKit-Image Van Der Walt et al.65 N/A SciKit-learn Pedregosa et al.66 N/A Meshlab Cignoni et al.67 N/A Napari Sofroniew et al.68 N/A Napari-process-points-and-surfaces Haase et al.69 N/A TrackPy Allan et al.70 N/A Affinity Designer Affinity affinity.studio LAS X Leica N/A Fusion Andor N/A Other SP8 Falcon laser scanning confocal microscope Leica N/A (Continued on next page) 18 Cell Reports 45, 117150, April 28, 2026 ..
Software:Article Title: Optogenetic mediated contractility enables reversible control of microglial morphology and migration in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins BODIPY 493/503 Thermo Fisher Scientific Cat#: D3922 N-Phenylthiourea (PTU) Sigma-Aldrich Cat#: P7629 Tricaine Sigma-Aldrich Cat#: A5040 CK-666 Sigma-Aldrich Cat#: SML0006 Blebbistatin MedChemExpress Cat#: HY-13441 Deposited data UAS:lyn-iLID-P2A-mScarlet-I-SspB-ArhGEF25b Addgene Cat#: 253351 Protrusion_morphology_seg_track_3D This study https://doi.org/10.5281/zenodo.18662631 Experimental models: Organisms/strains Danio rerio TgBAC(csf1ra:GAL4-VP16) Gray et al.55 ZFIN ID:ZDB-TGCONSTRCT-110707-1 Danio rerio Tg(mpeg1.1:GAL4-VP16) Ellet et al.56 ZFIN ID:ZDB-TGCONSTRCT-120117-3 Danio rerio Tg(UAS:lyn-tagRFPt) Möller et al.3 N/A Danio rerio Tg(NBT:ΔLexPR:LexOP:secA5-BFP) Mazaheri et al.2 ZFIN ID: ZDB-ALT-170110-1 Danio rerio TgBAC(p2y12:p2y12-GFP) Sieger et al.11 ZFIN ID: ZDB-TGCONSTRCT- 121109-1 Danio rerio Tg(UAS:Myl12.1-mNeonGreen) This study N/A Danio rerio Tg(UAS:LifeAct-mNeonGreen) This study N/A Danio rerio Tg(UAS:lyn-miRFP670) This study N/A Danio rerio Tg(UAS:lyn-iLID-P2AmScarlet-I-SspB-ArhGEF25b) This study N/A Software and algorithms FIJI Schindelin et al.57 N/A BigDataProcessor2 Tischer et al.58 N/A BioFormats Linkert et al.59 N/A Linear Stack Alignment with SIFT Fischler and Bolles60 Lowe61 N/A MTrackJ Meijering et al.62 N/A Trainable WEKA Segmentation Arganda-Carreras et al.63 N/A Huygens Professional SVI svi.nl/Huygens-Professional Ilastik Berg et al.64 N/A Imaris Oxford Instruments imaris.oxinst.com RStudio R project r-project.org Python Python python.org SciKit-Image Van Der Walt et al.65 N/A SciKit-learn Pedregosa et al.66 N/A Meshlab Cignoni et al.67 N/A Napari Sofroniew et al.68 N/A Napari-process-points-and-surfaces Haase et al.69 N/A TrackPy Allan et al.70 N/A Affinity Designer Affinity affinity.studio LAS X Leica N/A Fusion Andor N/A Other SP8 Falcon laser scanning confocal microscope Leica N/A (Continued on next page) 18 Cell Reports 45, 117150, April 28, 2026 ..
Microscopy:Article Title: Optogenetic mediated contractility enables reversible control of microglial morphology and migration in vivo.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins BODIPY 493/503 Thermo Fisher Scientific Cat#: D3922 N-Phenylthiourea (PTU) Sigma-Aldrich Cat#: P7629 Tricaine Sigma-Aldrich Cat#: A5040 CK-666 Sigma-Aldrich Cat#: SML0006 Blebbistatin MedChemExpress Cat#: HY-13441 Deposited data UAS:lyn-iLID-P2A-mScarlet-I-SspB-ArhGEF25b Addgene Cat#: 253351 Protrusion_morphology_seg_track_3D This study https://doi.org/10.5281/zenodo.18662631 Experimental models: Organisms/strains Danio rerio TgBAC(csf1ra:GAL4-VP16) Gray et al.55 ZFIN ID:ZDB-TGCONSTRCT-110707-1 Danio rerio Tg(mpeg1.1:GAL4-VP16) Ellet et al.56 ZFIN ID:ZDB-TGCONSTRCT-120117-3 Danio rerio Tg(UAS:lyn-tagRFPt) Möller et al.3 N/A Danio rerio Tg(NBT:ΔLexPR:LexOP:secA5-BFP) Mazaheri et al.2 ZFIN ID: ZDB-ALT-170110-1 Danio rerio TgBAC(p2y12:p2y12-GFP) Sieger et al.11 ZFIN ID: ZDB-TGCONSTRCT- 121109-1 Danio rerio Tg(UAS:Myl12.1-mNeonGreen) This study N/A Danio rerio Tg(UAS:LifeAct-mNeonGreen) This study N/A Danio rerio Tg(UAS:lyn-miRFP670) This study N/A Danio rerio Tg(UAS:lyn-iLID-P2AmScarlet-I-SspB-ArhGEF25b) This study N/A Software and algorithms FIJI Schindelin et al.57 N/A BigDataProcessor2 Tischer et al.58 N/A BioFormats Linkert et al.59 N/A Linear Stack Alignment with SIFT Fischler and Bolles60 Lowe61 N/A MTrackJ Meijering et al.62 N/A Trainable WEKA Segmentation Arganda-Carreras et al.63 N/A Huygens Professional SVI svi.nl/Huygens-Professional Ilastik Berg et al.64 N/A Imaris Oxford Instruments imaris.oxinst.com RStudio R project r-project.org Python Python python.org SciKit-Image Van Der Walt et al.65 N/A SciKit-learn Pedregosa et al.66 N/A Meshlab Cignoni et al.67 N/A Napari Sofroniew et al.68 N/A Napari-process-points-and-surfaces Haase et al.69 N/A TrackPy Allan et al.70 N/A Affinity Designer Affinity affinity.studio LAS X Leica N/A Fusion Andor N/A Other SP8 Falcon laser scanning confocal microscope Leica N/A (Continued on next page) 18 Cell Reports 45, 117150, April 28, 2026 ..
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